This protocol describes gating techniques to review T cells in mouse thymus, spleen, and blood. We defined the several T mobile populations according to extracellular and intracellular markers. Over the incubation of samples While using the antibody mix for extracellular staining, prepare all required buffers to detect intracellular cytokine output https://lustmap65320.hazeronwiki.com/7008580/how_and6_can_save_you_time_stress_and_money