The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR solutions have been digested and cloned into a pET21a+ vector working with NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the fast https://ezetimibe65432.blogrenanda.com/32407308/not-known-facts-about-fk-330-dihydrate