In Italy a nation-wide monitoring network was established in 2009 in response to significant honey bee colony mortality reported during 2008. The network comprised of approximately 100 apiaries located across Italy. Colonies were sampled four times per year. in order to assess the health status and to collect samples for pathogen. https://www.spidertattooz.com/The-GelBottle-Inc-Gel-Polish-Audrey-67oz-p17921/