I've never tried to freeze the actual biscuits (cooked). I have frozen the raw dough with success. You might give it a shot- my guess is they just could possibly be very dry right after defrosting but I am unsure our pups would care, ideal? In line with Dr. Muithi, https://titusyfmrc.pages10.com/dog-calming-treats-no-further-a-mystery-70231415